Changes in Pulmonary Interleukin-6 and Pulmonary Pathology in Neonatal Mice After Exposure to Hyperoxia and Ascorbate Treatment.

Chang,  Hye Young; Park,  Eun Hye; Oh,  Chang Hee; Park,  Min Soo; Namgung,  Ran; Park,  Kook In; Lee,  Chul; Han,  Dong Gwan

Original Article

Journal of the Korean Society of Neonatology 2001;8(1):103-109.
Published online May 1, 2001.

Changes in Pulmonary Interleukin-6 and Pulmonary Pathology in Neonatal Mice After Exposure to Hyperoxia and Ascorbate Treatment.

Hye Young Chang, Eun Hye Park, Chang Hee Oh, Min Soo Park, Ran Namgung, Kook In Park, Chul Lee, Dong Gwan Han

¹Department of Pediatrics, Yonsei University Collage of Medicine, Seoul, Korea.
²Department of Pediatrics, Kangdong Sacred Hospital, Seoul, Korea.
³Department of Pediatrics,College of Medicine, Kangnam Sacred Hospital,Hallym University, Seoul, Korea.

신생마우스에서 고농도 산소 노출 및 Ascorbate 처치에 따른 폐 조직과 폐내 Interleukin-6의 변화

^, ^, ^, ^, ^, ^, ^,

^¹
^²
^³

Abstract

PURPOSE
Levels of proinflammatory cytokines such as IL-1, IL-6 and TNF-alpha have been shown to be increased in the lung of adult mice after exposure to hyperoxia. Ascorbate, a non-enzymatic antioxidant, might have a protective effect against oxygen-induced lung injury. We examined effects of hyperoxia and ascorbate treatment on pulmonary IL-6 levels and pathology in the lungs of newborn mice.
METHODS
Neonatal mice were exposed to hyperoxia (95% O2) or room air (control) for 24 h or 48 h. Other groups, exposed to hyperoxia for up to 48 h, were injected intraperitoneally preventive doses (0.1 mg/10 g mouse weight) or therapeutic doses (1 mg/10 g mouse weight) of ascorbate or distilled water (placebo control) twice, immediately before and at 24 h of exposure to hyperoxia. IL-6 concentrations in the supernatant of minced lungs were measured by ELISA.
RESULTS
There were no pathologic changes in the lungs except mild emphysema and alveolar hemorrhage. The median pulmonary IL-6 concentration was significantly higher in mice exposed to hyperoxia for 24 h (P<0.05) and for 48 h (P<0.001) than room air controls. There was no difference in pulmonary IL-6 concentration between hyperoxia-exposed (48 h) mice treated with either preventive or therapeutic doses of ascorbate and placebo controls.
CONCLUSION
Neonatal mice exposed to hyperoxia for 24 h and 48 h showed higher pulmonary IL-6 concentrations than room air controls. Preventive or therapeutic doses of ascorbate had no effect on pulmonary IL-6 concentrations nor pulmonary pathology of neonatal mice exposed to hyperoxia for 48 h.

Key Words: Hyperoxia; Interleukin-6; Ascorbate